Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Cdc25A) Polyclonal Antibody, Unconjugated (ER1906-05) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Cdc25A) Polyclonal Antibody, Unconjugated (ER1906-05) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- Cdc25A (ER1906-05) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 61 kD| Product Name | Cdc25A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cdc 25a CDC25A2 Cell division cycle 25 homolog A (S. pombe) Cell division cycle 25A Cell division cycle 25A isoform a Cell division cycle 25A isoform b D9Ertd393e Dual specificity phosphatase CDC25A EC 3.1.3.48 M phase inducer phosphatase 1 MGC115549 CDC25A CDC25A2 CAG isoform M-phase inducer phosphatase 1 MPIP1_HUMAN. |
| Product description | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | KLH conjugated synthetic peptide derived from human cdc25A 451-524/524 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Cdc 25a
CDC25A2
Cell division cycle 25 homolog A (S. pombe) Cell division cycle 25A Cell division cycle 25A isoform a Cell division cycle 25A isoform b D9Ertd393e Dual specificity phosphatase CDC25A EC 3.1.3.48 M phase inducer phosphatase 1 MGC115549 CDC25A CDC25A2 CAG isoform M-phase inducer phosphatase 1 MPIP1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| Cellular Localization | Cytosol, Nucleus. |
| SwissProt ID | P30304 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Cdc25A) Polyclonal Antibody, Unconjugated (ER1906-05) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Cdc25A) Polyclonal Antibody, Unconjugated (ER1906-05) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- Cdc25A (ER1906-05) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 61 kD| Positive Control | Mouse brain tissue, Rat small intestine tissue, Testis (Mouse) Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
