Application
Fig1: Sample: HL-60(Human) Cell Lysate at 40 ug Primary: Anti-CEAcam8 (ER1906-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 75 kD| Product Name | CEAcam8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carcinoembryonic Antigen CGM6 Carcinoembryonic antigen gene family member 6 Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 CD 66b CD 67 CD66b antigen CD67 CD67 antigen CEACAM 8 CEACAM8 CGM 6 CGM6 NCA 95 NCA95 Nonspecific cross reacting antigen NCA 95 Non-specific -reacting antigen NCA-95 Nonspecific cross reacting antigen NCA95 CEAM8_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 8 CD66b. |
| Product description | The carcino-embryonic antigen-related cell adhesion molecule (CEACAM)8, often better known as CD66b, encodes a glycosylphosphatidylinositol (GPI)-linked glycoprotein, which is exclusively expressed by human granulocytes. CEACAM8 belongs to the carcinoembryonic antigen (CEA) family of the immunoglobulin superfamily. CEACAMs are involved in various intercellular-adhesion and cellular signaling-mediated effects modulating immune responses which are associated with the binding of pathogens, inflammation as well as growth and/or differentiation of normal and cancerous cells. CEACAM8 is stored in specific vesicles of granulocytes and acts as a marker for specific vesicles for exocytosis [8]. Secretion has been shown to be induced by Phorbol-12-myristate-13-acetate (PMA). Interestingly, no homolog for CEACAM8 has been identified in rodents, suggesting that there may be a strong selection pressure (e.g., exposure to microorganisms or parasites) during the evolution of molecules of the CEA family. The soluble form of CEACAM8 binds to CEACAM1, a trans-membrane-bound molecule expressed by certain normal epithelial, endothelial, different leukocyte subpopulations and some cancer cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human CEAcam8 51-150/349 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Carcinoembryonic Antigen CGM6
Carcinoembryonic antigen gene family member 6 Carcinoembryonic Antigen Related Cell Adhesion Molecule 8 CD 66b CD 67 CD66b antigen CD67 CD67 antigen CEACAM 8 CEACAM8 CGM 6 CGM6 NCA 95 NCA95 Nonspecific cross reacting antigen NCA 95 Non-specific -reacting antigen NCA-95 Nonspecific cross reacting antigen NCA95 CEAM8_HUMAN Carcinoembryonic antigen-related cell adhesion molecule 8 CD66b. |
|---|---|
| Molecular Weight(MW) | 38 KDa |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| SwissProt ID | P31997 |
|---|
Application
Fig1: Sample: HL-60(Human) Cell Lysate at 40 ug Primary: Anti-CEAcam8 (ER1906-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 75 kD| Positive Control | Expressed in leukocytes of chronic myeloid Leukemia patients and bone marrow. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
