Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- CENPH (ER1906-10) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD| Product Name | CENPH |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ENP H CENP-H CENPH CENPH_HUMAN Centromere protein H ICEN35 Interphase centromere complex protein 35 Kinetochore protein CENP H NNF1 NNF1, MIND kinetochore complex component, homolog PMF1. |
| Product description | Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. |
| Immunogen | KLH conjugated synthetic peptide derived from human CENPH 41-140/247 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | ENP H
CENP-H
CENPH
CENPH_HUMAN
Centromere protein H
ICEN35
Interphase centromere complex protein 35
Kinetochore protein CENP H
NNF1
NNF1 MIND kinetochore complex component homolog PMF1. |
|---|---|
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Note=Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase. |
| SwissProt ID | Q9H3R5 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- CENPH (ER1906-10) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
