Application
Fig1: Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CEP192 Polyclonal Antibody, Unconjugated(ER1906-15) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-CEP192 (ER1906-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 213 kD Observed band size: 150 kDApplication
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-CEP192 (ER1906-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 213 kD Observed band size: 150 kD| Product Name | CEP192 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Centrosomal protein 192kDa Centrosomal protein of 192 kDa Cep192 KIAA1569 PP8407 PPP1R62 CE192_HUMAN Protein phosphatase 1, regulatory subunit 62. |
| Product description | Centrosomal protein of 192 kDa, also known as Cep192, is a protein that in humans is encoded by the CEP192 gene. It is the homolog of the C. elegans and D. melanogaster gene SPD-2. Cep192 is a major regulator of pericentriolar material recruitment, centrosome maturation, and centriole duplication in mammalian cells. It stimulates the formation of the scaffolding upon which gamma tubulin ring complexes and other proteins involved in microtubule nucleation and spindle assembly become functional during mitosis. |
| Immunogen | KLH conjugated synthetic peptide derived from human CEP192 51-150/1941 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Centrosomal protein 192kDa
Centrosomal protein of 192 kDa
Cep192
KIAA1569
PP8407
PPP1R62
CE192_HUMAN
Protein phosphatase 1 regulatory subunit 62. |
|---|---|
| Molecular Weight(MW) | 213 KDa |
| Cellular Localization | Required for mitotic centrosome and spindle assembly. Appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication. |
| SwissProt ID | Q8TEP8 |
|---|
Application
Fig1: Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CEP192 Polyclonal Antibody, Unconjugated(ER1906-15) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-CEP192 (ER1906-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 213 kD Observed band size: 150 kD
Application
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-CEP192 (ER1906-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 213 kD Observed band size: 150 kD| Positive Control | Human liver cancer tissue, Spleen (Mouse) Lysate, Testis (Mouse) Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
