Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-CERD4(ER1906-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 48 kDApplication
Fig2: Sample: testis (Mouse) Lysate at 40 ug Primary: Anti-CERD4(ER1906-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 48 kD| Product Name | CERD4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2810403B03Rik BAF45C BRG1-associated factor 45C cer-d4 homolog CERD4 D4, zinc and double PHD fingers, family 3 DPF 3 Dpf3 DPF3_HUMAN FLJ 14079 FLJ14079 Zinc finger protein cer-d4 Zinc finger protein DPF3. |
| Product description | Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development. |
| Immunogen | KLH conjugated synthetic peptide derived from human CERD4 1-100/378 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | 2810403B03Rik
BAF45C
BRG1-associated factor 45C
cer-d4 homolog
CERD4
D4 zinc and double PHD fingers family 3 DPF 3 Dpf3 DPF3_HUMAN FLJ 14079 FLJ14079 Zinc finger protein cer-d4 Zinc finger protein DPF3. |
|---|---|
| Molecular Weight(MW) | 43 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q92784 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-CERD4(ER1906-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 48 kD
Application
Fig2: Sample: testis (Mouse) Lysate at 40 ug Primary: Anti-CERD4(ER1906-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 48 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
