Application
Fig1: Protein: Hela(human) lysate at 40ug; Primary: rabbit Anti-CFC1 (ER1906-18) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 15 kD Observed band size: 15 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CFC1) Polyclonal Antibody, Unconjugated (ER1906-18) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | CFC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CFC 1 CFC1 CFC1_HUMAN CFC1B CR 1 Cripto 1 Cripto Cripto FRL 1 cryptic family 1 CRYPTIC Cryptic family 1 Cryptic family protein 1 Cryptic gene Cryptic protein DTGA2 FLJ77897 FRL 1 HTX2 MGC133213. |
| Product description | NODAL coreceptor involved in the correct establishment of the left-right axis. May play a role in mesoderm and/or neural patterning during gastrulation. |
| Immunogen | KLH conjugated synthetic peptide derived from human CFC1 1-100/223 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CFC 1 CFC1 CFC1_HUMAN CFC1B CR 1 Cripto 1 Cripto Cripto FRL 1 cryptic family 1 CRYPTIC Cryptic family 1 Cryptic family protein 1 Cryptic gene Cryptic protein DTGA2 FLJ77897 FRL 1 HTX2 MGC133213. |
|---|---|
| Molecular Weight(MW) | 15 KDa |
| Cellular Localization | Cell membrane. Secreted. Does not exhibit a typical GPI-signal sequence. The C-ter hydrophilic extension of the GPI-signal sequence reduces the efficiency of processing and could lead to the production of an secreted unprocessed form. This extension is found only in primates. |
| SwissProt ID | P0CG37 |
|---|
Application
Fig1: Protein: Hela(human) lysate at 40ug; Primary: rabbit Anti-CFC1 (ER1906-18) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 15 kD Observed band size: 15 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CFC1) Polyclonal Antibody, Unconjugated (ER1906-18) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
