Application
Fig1: Sample: A549 (human)Cell Lysate at 40 ug Primary: Anti-CHST10(ER1906-27) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 52 kDApplication
Fig2: Sample: 293T (human)cell Lysate at 40 ug Primary: Anti- CHST10(ER1906-27)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42kD Observed band size: 42 kD| Product Name | CHST10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carbohydrate sulfotransferase 10 CHST10 CHSTA_HUMAN EC 2.8.2.- HNK-1 sulfotransferase HNK-1ST HNK1ST huHNK-1ST MGC17148 OTTHUMP00000203188. |
| Product description | Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human CHST10 281-356/356 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Carbohydrate sulfotransferase 10 CHST10 CHSTA_HUMAN EC 2.8.2.- HNK-1 sulfotransferase HNK-1ST HNK1ST huHNK-1ST MGC17148 OTTHUMP00000203188. |
|---|---|
| Molecular Weight(MW) | 42 KDa |
| Cellular Localization | Golgi apparatus membrane. |
| SwissProt ID | O43529 |
|---|
Application
Fig1: Sample: A549 (human)Cell Lysate at 40 ug Primary: Anti-CHST10(ER1906-27) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 52 kD
Application
Fig2: Sample: 293T (human)cell Lysate at 40 ug Primary: Anti- CHST10(ER1906-27)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42kD Observed band size: 42 kD| Positive Control | In fetal tissues, it is predominantly expressed in brain, and weakly expressed in lung, kidney and liver. In adult, it is highly expressed in brain, testis, ovary, expressed at intermediate level in heart, pancreas, skeletal muscle, spleen and thymus, and weakly expressed in other tissues. In brain, it is expressed at higher level in the frontal lobe. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
