Application
Fig1: Sample: A549 (human)Cell Lysate at 40 ug Primary: Anti-CHST(ER1906-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-CHST3(ER1906-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 60 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CHST3) Polyclonal Antibody, Unconjugated (ER1906-28) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | CHST3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C6ST 1 C6ST C6ST-1 C6ST1 Carbohydrate (chondroitin 6) sulfotransferase 3 Carbohydrate sulfotransferase 3 Chondroitin 6 O sulfotransferase 1 Chondroitin 6 sulfotransferase Chondroitin 6-O-sulfotransferase 1 Chondroitin 6-sulfotransferase CHST 3 Chst3 CHST3_HUMAN Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 0 Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0 GST 0 GST-0 GST0. |
| Product description | Catalyzes the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen. |
| Immunogen | KLH conjugated synthetic peptide derived from human CHST3 21-120/479 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | C6ST 1 C6ST C6ST-1 C6ST1 Carbohydrate (chondroitin 6) sulfotransferase 3 Carbohydrate sulfotransferase 3 Chondroitin 6 O sulfotransferase 1 Chondroitin 6 sulfotransferase Chondroitin 6-O-sulfotransferase 1 Chondroitin 6-sulfotransferase CHST 3 Chst3 CHST3_HUMAN Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 0 Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0 GST 0 GST-0 GST0. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Golgi apparatus membrane. |
| SwissProt ID | Q7LGC8 |
|---|
Application
Fig1: Sample: A549 (human)Cell Lysate at 40 ug Primary: Anti-CHST(ER1906-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-CHST3(ER1906-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 60 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CHST3) Polyclonal Antibody, Unconjugated (ER1906-28) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
