Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CHST7) Polyclonal Antibody, Unconjugated (ER1906-30) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Ovary (Mouse) Lysate at 40 ug Primary: Anti- CHST7 (ER1906-30) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD| Product Name | CHST7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 6 O sulfotransferase 5 C6ST 2 C6ST2 Carbohydrate sulfotransferase 7 Chondroitin 6 sulfotransferase 2 CHST 7 CHST7_HUMAN CHST7 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7 Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 5 GlcNAc6ST 4 Gn6st-4 GST 5 N acetylglucosamine 6 O sulfotransferase 1. |
| Product description | CHST7 belongs to the sulfotransferase family. Sulfotransferases generate sulfated glycosaminoglycan (GAG) moities during chondroitin sulfate biosynthesis. They create considerable structural diversity among chondroitin sulfates by transferring sulfate with remarkable specificity for the underlying oligosaccharide substrate. This protein mainly transfers sulfate to N-acetylgalactosamine. The regulated expression of each member of the family may be an important determinant of sulfated GAGs expression and the associated function of chondroitin sulfates as regulators of many biologic processes. |
| Immunogen | KLH conjugated synthetic peptide derived from human CHST7 101-200/486 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | 6 O sulfotransferase 5 C6ST 2 C6ST2 Carbohydrate sulfotransferase 7 Chondroitin 6 sulfotransferase 2 CHST 7 CHST7_HUMAN CHST7 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7 Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 5 GlcNAc6ST 4 Gn6st-4 GST 5 N acetylglucosamine 6 O sulfotransferase 1. |
|---|---|
| Molecular Weight(MW) | 54 KDa |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
| SwissProt ID | Q9NS84 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CHST7) Polyclonal Antibody, Unconjugated (ER1906-30) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Ovary (Mouse) Lysate at 40 ug Primary: Anti- CHST7 (ER1906-30) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD| Positive Control | Widely expressed. Highly expressed in heart, spleen, liver and ovary. Expressed at lower level in brain, placenta, thyroid, spinal cord and peripheral blood leukocytes. Not expressed in adult skin. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
