Application
Fig1: Sample: Epididymis (Mouse) Lysate at 40 ug Primary: Anti-CIDEC (ER1906-31) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (ER1906-31) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(ER1906-31) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(ER1906-31) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (ER1906-31) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | CIDEC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cell Death Activator Cell death activator CIDE-3 Cell Death Inducing DFFA Like Effector C Cell death inducing DFFA like effector protein C Cell death-inducing DFFA-like effector protein C CIDE 3 CIDE3 CIDE C CIDEC_HUMAN Fat specific protein 27 Fat-specific protein FSP27 homolog FLJ20871 FSP27. |
| Product description | May act as a CEBPB coactivator in white adipose tissueto control the expression of a subset of CEBPB downstream targetgenes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH (Bysimilarity). Binds to lipid droplets and regulates theirenlargement, thereby restricting lipolysis and favoring storage. Atfocal contact sites between lipid droplets, promotes directionalnet neutral lipid transfer from the smaller to larger lipiddroplets. The transfer direction may be driven by the internalpressure difference between the contacting lipid droplet pair. Whenoverexpressed in preadipocytes, induces apoptosis or increases cellsusceptibility to apoptosis induced by serum deprivation or TGFBtreatment. As mature adipocytes, that express high CIDEC levels,are quite resistant to apoptotic stimuli, the physiologicalsignificance of its role in apoptosis is unclear. |
| Immunogen | KLH conjugated synthetic peptide derived from human CIDEC 101-200/238 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPig |
| Concentration | 1mg/ml |
| Alternative Names | Cell Death Activator Cell death activator CIDE-3 Cell Death Inducing DFFA Like Effector C Cell death inducing DFFA like effector protein C Cell death-inducing DFFA-like effector protein C CIDE 3 CIDE3 CIDE C CIDEC_HUMAN Fat specific protein 27 Fat-specific protein FSP27 homolog FLJ20871 FSP27. |
|---|---|
| Molecular Weight(MW) | 27 KDa |
| Cellular Localization | Nucleus (By similarity). Endoplasmicreticulum (By similarity). Lipid droplet. Note=Diffuses quickly onlipid droplet surface, but becomes trapped and clustered at lipiddroplet contact sites, thereby enabling its rapid enrichment atlipid droplet contact sites. |
| SwissProt ID | Q96AQ7 |
|---|
Application
Fig1: Sample: Epididymis (Mouse) Lysate at 40 ug Primary: Anti-CIDEC (ER1906-31) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (ER1906-31) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(ER1906-31) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CIDEC Polyclonal Antibody, Unconjugated(ER1906-31) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated (ER1906-31) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Expressed mainly in adipose tissue, smallintestine, heart, colon and stomach and, at lower levels, in brain,kidney and liver. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
