Application
Fig1: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-CILP (ER1906-32) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 132 kD Observed band size: 132 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CILP) Polyclonal Antibody, Unconjugated (ER1906-32) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | CILP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cartilage intermediate layer protein 1 C1 Cartilage intermediate layer protein 1 C2 cartilage intermediate layer protein cartilage intermediate layer protein, nucleotide pyrophosphohydrolase Cartilage intermediate-layer protein CILP 1 CILP CILP-1 CILP1_HUMAN HGNC:1980 HsT18872. |
| Product description | Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. |
| Immunogen | KLH conjugated synthetic peptide derived from human CILP 1051-1184/1184 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Cartilage intermediate layer protein 1 C1
Cartilage intermediate layer protein 1 C2
cartilage intermediate layer protein
cartilage intermediate layer protein nucleotide pyrophosphohydrolase Cartilage intermediate-layer protein CILP 1 CILP CILP-1 CILP1_HUMAN HGNC:1980 HsT18872. |
|---|---|
| Molecular Weight(MW) | 132 KDa |
| Cellular Localization | Secreted > extracellular space > extracellular matrix. |
| SwissProt ID | O75339 |
|---|
Application
Fig1: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-CILP (ER1906-32) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 132 kD Observed band size: 132 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CILP) Polyclonal Antibody, Unconjugated (ER1906-32) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Specifically expressed in cartilage. Localizes in the intermediates layer of articular cartilage but neither in the superficial nor in the deepest regions. Specifically and highly expressed in intervertebral disk tissue. Expression increases with aging in hip articular cartilage. Overexpressed in articular hyaline cartilage from patients with calcium pyrophosphate dihydrate crystal deposition disease (CPPD). Expression in intervertebral disk tissue from individuals with lumbar disk disease increases as disk degeneration progresses. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
