Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CITED1 ) Polyclonal Antibody, Unconjugated (ER1906-33) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-CITED1 (ER1906-33) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD| Product Name | CITED1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cbp/p300-interacting transactivator 1 Cbp/p300-interacting transactivator with GLU/ASP-rich C-terminal domain, 1 Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1 melanocyte specific gene 1 Melanocyte specific protein 1 MSG1. |
| Product description | Transcriptional coactivator of the p300/CBP-mediated trancription complex. Enhances SMAD-mediated transcription by strengthening the functional link between the DNA-binding SMAD transcription factors and the p300/CBP transcription coactivator complex. Stimulates estrogen-dependent transactivation activity mediated by estrogen receptors signaling; stabilizes the interaction of estrogen receptor ESR1 and histone acetyltransferase EP300. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Induces transcription from estrogen-responsive promoters and protection against cell death. Potentiates EGR2-mediated transcriptional activation activity from the ERBB2 promoter. Acts as an inhibitor of osteoblastic mineralization through a cAMP-dependent parathyroid hormone receptor signaling. May play a role in pigmentation of melanocytes. Associates with chromatin to the estrogen-responsive TGF-alpha promoter region in a estrogen-dependent manner. |
| Immunogen | KLH conjugated synthetic peptide derived from human CITED1/ABCC1 121-193/193 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cbp/p300-interacting transactivator 1
Cbp/p300-interacting transactivator with GLU/ASP-rich C-terminal domain 1 Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1 melanocyte specific gene 1 Melanocyte specific protein 1 MSG1. |
|---|---|
| Molecular Weight(MW) | 20 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm by a nuclear export signal and (NES) in a CRM1-dependent manner. |
| SwissProt ID | Q99966 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CITED1 ) Polyclonal Antibody, Unconjugated (ER1906-33) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-CITED1 (ER1906-33) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD| Positive Control | Expressed only in melanocytes and testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
