Application
Fig1: Western blot analysis of Tryptophanyl tRNA synthetase / WRS on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Tryptophanyl tRNA synthetase / WRS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-73, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig3: Flow cytometric analysis of Tryptophanyl tRNA synthetase / WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-73, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Tryptophanyl tRNA synthetase / WRS [JE48-08] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gamma 2 antibody GAMMA-2 antibody Gamma2 antibody hWRS antibody IFI 53 antibody IFI53 antibody IFP 53 antibody IFP53 antibody Interferon induced protein 53 antibody Interferon-induced protein 53 antibody SYWC_HUMAN antibody T2-TrpRS antibody TrpRS antibody Tryptophan tRNA ligase, cytoplasmic antibody Tryptophan tRNA ligase 1 cytoplasmic antibody Tryptophan tRNA ligase antibody Tryptophan--tRNA ligase antibody Tryptophanyl tRNA synthetase antibody Tryptophanyl tRNA synthetase cytoplasmic antibody WARS antibody WARS protein antibody WRS antibody |
| Product description | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. |
| Immunogen | Synthetic peptide within C-terminal human Tryptophanyl tRNA synthetase/WRS. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000: IHC:1:50-1:200: FC:1:50-1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Gamma 2 antibody
GAMMA-2 antibody
Gamma2 antibody
hWRS antibody
IFI 53 antibody
IFI53 antibody
IFP 53 antibody
IFP53 antibody
Interferon induced protein 53 antibody
Interferon-induced protein 53 antibody
SYWC_HUMAN antibody
T2-TrpRS antibody
TrpRS antibody
Tryptophan tRNA ligase cytoplasmic antibody Tryptophan tRNA ligase 1 cytoplasmic antibody Tryptophan tRNA ligase antibody Tryptophan--tRNA ligase antibody Tryptophanyl tRNA synthetase antibody Tryptophanyl tRNA synthetase cytoplasmic antibody WARS antibody WARS protein antibody WRS antibody |
|---|---|
| Molecular Weight(MW) | 49/53 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P23381 |
|---|
Application
Fig1: Western blot analysis of Tryptophanyl tRNA synthetase / WRS on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Tryptophanyl tRNA synthetase / WRS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-73, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Flow cytometric analysis of Tryptophanyl tRNA synthetase / WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-73, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | K562 cell lysate, human lung tissue, A549. |
|---|---|
| Application Notes | WB:1:500-1:1,000: IHC:1:50-1:200: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
