Application
Fig1: Sample: A431 Cell Lysate at 30 ug Primary: Anti- CK II alpha (ER1906-34) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kDApplication
Fig2: Sample: HepG2 Cell Lysate at 30 ug Primary: Anti-CK II alpha (ER1906-34) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD| Product Name | CK II alpha |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STK Casein kinase II Alpha CKII alpha Serine/Threonine Protein Kinase II Alpha CKⅡalpha Casein kinase 2 alpha 1 polypeptide Casein kinase II alpha 1 subunit Casein kinase II alpha chain Casein kinase II alpha subunit Casein kinase II subunit alpha Casein kinase II alpha 1 CK II alpha CKIIalpha CSK21_HUMAN CK II CK2 alpha CK2 catalytic subunit alpha CK2A1 CKII CSNK2A1. |
| Product description | Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation. |
| Immunogen | KLH conjugated synthetic peptide derived from human CK II alpha 201-300/391 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | STK Casein kinase II Alpha CKII alpha Serine/Threonine Protein Kinase II Alpha CKⅡalpha Casein kinase 2 alpha 1 polypeptide Casein kinase II alpha 1 subunit Casein kinase II alpha chain Casein kinase II alpha subunit Casein kinase II subunit alpha Casein kinase II alpha 1 CK II alpha CKIIalpha CSK21_HUMAN CK II CK2 alpha CK2 catalytic subunit alpha CK2A1 CKII CSNK2A1. |
|---|---|
| Molecular Weight(MW) | 45 KDa |
| SwissProt ID | P68400 |
|---|
Application
Fig1: Sample: A431 Cell Lysate at 30 ug Primary: Anti- CK II alpha (ER1906-34) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD
Application
Fig2: Sample: HepG2 Cell Lysate at 30 ug Primary: Anti-CK II alpha (ER1906-34) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
