Application
Fig1: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CKMT/Creatine kinase MT Polyclonal Antibody, Unconjugated(ER1906-35) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Small intestine (Mouse) Lysate at 40 ug Primary: Anti-CKMT (ER1906-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kDApplication
Fig3: Sample: Large intestine (Rat) Lysate at 40 ug Primary: Anti-CKMT (ER1906-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD| Product Name | CKMT |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acidic type mitochondrial creatine kinase CKMT CKMT1 CKMT1A CKMT1B Creatine kinase mitochondrial 1 ubiquitous Creatine kinase ubiquitous mitochondrial Mia CK Ubiquitous mitochondrial creatine kinase UMTCK KCRU_HUMAN. |
| Product description | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. |
| Immunogen | KLH conjugated synthetic peptide derived from human CKMT 115-220/417 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Acidic type mitochondrial creatine kinase CKMT CKMT1 CKMT1A CKMT1B Creatine kinase mitochondrial 1 ubiquitous Creatine kinase ubiquitous mitochondrial Mia CK Ubiquitous mitochondrial creatine kinase UMTCK KCRU_HUMAN. |
|---|---|
| Molecular Weight(MW) | 43 KDa |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. |
| SwissProt ID | P12532 |
|---|
Application
Fig1: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CKMT/Creatine kinase MT Polyclonal Antibody, Unconjugated(ER1906-35) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Small intestine (Mouse) Lysate at 40 ug Primary: Anti-CKMT (ER1906-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD
Application
Fig3: Sample: Large intestine (Rat) Lysate at 40 ug Primary: Anti-CKMT (ER1906-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
