Application
Fig1: Sample: Olfactory bulb (Mouse) Lysate at 40 ug Primary: Anti-Clathrin heavy chain (ER1906-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 192 kD Observed band size: 192 kD| Product Name | Clathrin heavy chain |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHC17 Clathrin heavy chain 1 Clathrin heavy chain 2 Clathrin heavy chain Clathrin heavy chain like 1 Clathrin heavy chain on chromosome 17 Clathrin heavy polypeptide CLH 17 CLH 22 CLH-17 CLH1_HUMAN CLH17 CLH22 CLTC CLTCL CLTCL1 CLTCL2 CLTD Hc KIAA0034. |
| Product description | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation |
| Immunogen | KLH conjugated synthetic peptide derived from human Clathrin heavy chain. 401-500/1675 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitQueen BeeOtherFrog |
| Concentration | 1mg/ml |
| Alternative Names | CHC17 Clathrin heavy chain 1 Clathrin heavy chain 2 Clathrin heavy chain Clathrin heavy chain like 1 Clathrin heavy chain on chromosome 17 Clathrin heavy polypeptide CLH 17 CLH 22 CLH-17 CLH1_HUMAN CLH17 CLH22 CLTC CLTCL CLTCL1 CLTCL2 CLTD Hc KIAA0034. |
|---|---|
| Molecular Weight(MW) | 192 KDa |
| Cellular Localization | Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| SwissProt ID | Q00610 |
|---|
Application
Fig1: Sample: Olfactory bulb (Mouse) Lysate at 40 ug Primary: Anti-Clathrin heavy chain (ER1906-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 192 kD Observed band size: 192 kD| Positive Control | Olfactory bulb (Mouse) Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
