Application
Fig1: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(ER1906-40) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(ER1906-40) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CLEC16A) Polyclonal Antibody, Unconjugated (ER1906-40) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig4: Sample: k562 (human)Cell Lysate at 40 ug Primary: Anti-CLEC16A(ER1906-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kDApplication
Fig5: Sample: K562(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug U-2OS(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug Primary: Anti- CLEC16A (ER1906-40) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD| Product Name | CLEC16A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein CLEC16A C type lectin domain family 16 member A CL16A Gop 1 Gop1 CL16A_HUMAN； KIAA0350 MGC111457. |
| Immunogen | KLH conjugated synthetic peptide derived from human CLEC16A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCow |
| Concentration | 1mg/ml |
| Alternative Names | Protein CLEC16A C type lectin domain family 16 member A CL16A Gop 1 Gop1 CL16A_HUMAN； KIAA0350 MGC111457. |
|---|---|
| Molecular Weight(MW) | 118 KDa |
| Cellular Localization | Cell membrane, Cytoplasmic. |
| SwissProt ID | Q2KHT3 |
|---|
Application
Fig1: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(ER1906-40) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(ER1906-40) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CLEC16A) Polyclonal Antibody, Unconjugated (ER1906-40) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig4: Sample: k562 (human)Cell Lysate at 40 ug Primary: Anti-CLEC16A(ER1906-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD
Application
Fig5: Sample: K562(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug U-2OS(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug Primary: Anti- CLEC16A (ER1906-40) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD| Positive Control | Almost exclusively expressed in immune cells, including dendritic cells, B-lymphocytes and natural killer cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
