Application
Fig1: Cell: U937. Concentration: 1:100. Incubation: 40 minutes, room temperature. Host/Blank: U937 cells. Flow cytometric analysis of Rabbit Anti-CLEC5A antibody (ER1906-41) (green) compared with control in the absence of primary antibody (blue) followed by U937 cells. secondary antibody: Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC)Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-CLEC5A(ER1906-41) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD| Product Name | CLEC5A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A1 catalytic A2 catalytic Alkali myosin light chain 1 Alkali myosin light chain 3 C36E6.3 CMH10 DKFZp779C0562 KMLC MLC 2 MLC 2v MLC MLC1F MLC2 MLC3F C-type lectin domain family 5 member A C type lectin domain family 5 member A C-type lectin superfamily member 5 CLEC5 A CLEC5A CLECSF 5 CLECSF5 MDL 1 MDL-1 MDL1 CLC5A_MOUSE Myeloid DAP12-associating lectin 1 Myeloid DAP12 associating lectin. |
| Product description | Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through phosphylation of TYROBP, this interaction does not result in viral entry but stimulates proinflammatory cytokine release. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CLEC5A 101-190/190 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | A1 catalytic A2 catalytic Alkali myosin light chain 1 Alkali myosin light chain 3 C36E6.3 CMH10 DKFZp779C0562 KMLC MLC 2 MLC 2v MLC MLC1F MLC2 MLC3F C-type lectin domain family 5 member A C type lectin domain family 5 member A C-type lectin superfamily member 5 CLEC5 A CLEC5A CLECSF 5 CLECSF5 MDL 1 MDL-1 MDL1 CLC5A_MOUSE Myeloid DAP12-associating lectin 1 Myeloid DAP12 associating lectin. |
|---|---|
| Molecular Weight(MW) | 22 KDa |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. |
| SwissProt ID | Q9R007 |
|---|
Application
Fig1: Cell: U937. Concentration: 1:100. Incubation: 40 minutes, room temperature. Host/Blank: U937 cells. Flow cytometric analysis of Rabbit Anti-CLEC5A antibody (ER1906-41) (green) compared with control in the absence of primary antibody (blue) followed by U937 cells. secondary antibody: Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC)
Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-CLEC5A(ER1906-41) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD| Positive Control | Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U937 and MonoMac6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
