Application
Fig1: Sample: 293T (human)Cell Lysate at 40 ug A549 (human)Cell Lysate at 40 ug MCF-7 (human)Cell Lysate at 40 ug HepG2 (human)Cell Lysate at 40 ug Placenta (mouse) Lysate at 40 ug MG63 (human)Cell Lysate at 40 ug Primary: Anti- Collagen II (ER1906-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 120 kDIF
C. The expression level of collagen II was determined by immunofluorescence assay.| Product Name | Collagen II |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. |
| Immunogen | KLH conjugated synthetic peptide derived from human Collagen II 1201-1300/1487 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIFIHC-PWB |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
|
| Species Reactivity | ChickenCowDogGuinea PigHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Alternative Names | Collagen II alpha 1
COL2A1
COL2A1 protein
collagen type II alpha 1 collagen alpha-1(II) type II collagen alpha-1 type II collagen alpha1 type II collagen Col2a1 AOM Cartilage collagen Chondrocalcin COL11A3 Collagen alpha 1(II) chain precursor Collagen II alpha 1 polypeptide Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital) MGC131516 SEDC Collagen alpha-1(II) chain Alpha-1 type II collagen CO2A1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 117 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | P02458 |
|---|
Application
Fig1: Sample: 293T (human)Cell Lysate at 40 ug A549 (human)Cell Lysate at 40 ug MCF-7 (human)Cell Lysate at 40 ug HepG2 (human)Cell Lysate at 40 ug Placenta (mouse) Lysate at 40 ug MG63 (human)Cell Lysate at 40 ug Primary: Anti- Collagen II (ER1906-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 120 kD
IF
C. The expression level of collagen II was determined by immunofluorescence assay.| Positive Control | Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
