Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse ear); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (ER1906-49) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated(ER1906-49) 1:100, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Sample: bone(Mouse) Lysate at 40 ug Primary: Anti-Collagen II(ER1906-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 134 kDApplication
Fig4: Sample: Embryo (Mouse) Lysate at 40 ug MG63 (Human) Cell Lysate at 30 ug Primary: Anti-Collagen II (ER1906-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 117 kDApplication
Fig5: Sample: 293T(Human) Cell Lysate at 30 ug A549 (Human) Cell Lysate at 30 ug LOVO (Human) Cell Lysate at 40 ug MCF-7 (Human) Cell Lysate at 30 ug HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Collagen II (ER1906-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 117 kDIF
C. The expression level of collagen II was determined by immunofluorescence assay.| Product Name | Collagen II |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. |
| Immunogen | KLH conjugated synthetic peptide derived from human Collagen II 231-330/1487 |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIFIHC-PWB |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: ICC:1:100-500: |
|
| Species Reactivity | ChickenCowDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ |
| Alternative Names | Collagen II alpha 1
COL2A1
COL2A1 protein collagen type II alpha 1 collagen alpha-1(II) type II collagen alpha-1 type II collagen alpha1 type II collagen Col2a1 AOM Cartilage collagen Chondrocalcin COL11A3 Collagen alpha 1(II) chain precursor Collagen II alpha 1 polypeptide Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital) MGC131516 SEDC CO2A1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 117 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | P02458 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse ear); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (ER1906-49) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated(ER1906-49) 1:100, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Sample: bone(Mouse) Lysate at 40 ug Primary: Anti-Collagen II(ER1906-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 134 kD
Application
Fig4: Sample: Embryo (Mouse) Lysate at 40 ug MG63 (Human) Cell Lysate at 30 ug Primary: Anti-Collagen II (ER1906-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 117 kD
Application
Fig5: Sample: 293T(Human) Cell Lysate at 30 ug A549 (Human) Cell Lysate at 30 ug LOVO (Human) Cell Lysate at 40 ug MCF-7 (Human) Cell Lysate at 30 ug HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Collagen II (ER1906-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 117 kD
IF
C. The expression level of collagen II was determined by immunofluorescence assay.| Positive Control | Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: ICC:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
