Application
Fig1: Western blot analysis of APRIL/TNFSF13 on Hela cell and A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining of APRIL/TNFSF13 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-89, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).| Product Name | APRIL/TNFSF13 [JE49-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A proliferation inducing ligand antibody A proliferation-inducing ligand antibody APRIL antibody CD256 antibody TALL-2 antibody TALL2 antibody TNF related death ligand antibody TNF- and APOL-related leukocyte expressed ligand 2 antibody TNF-related death ligand 1 antibody TNF13_HUMAN antibody TNFSF13 antibody TRDL-1 antibody TRDL1 antibody Tumor necrosis factor (ligand) superfamily, member 13 antibody Tumor necrosis factor ligand superfamily member 13 antibody Tumor necrosis factor like protein ZTNF2 antibody Tumor necrosis factor related death ligand 1 antibody UNQ383/PRO715 antibody ZTNF2 antibody |
| Product description | This gene encodes a member of the tumor necrosis factor superfamily. It encodes a hybrid protein composed of the cytoplasmic and transmembrane domains of family member 12 fused to the C-terminal domain of family member 13. Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. The hybrid protein is membrane anchored and presents the receptor-binding domain of family member 13 at the cell surface. It stimulates cycling in T- and B-lymphoma cell lines. |
| Immunogen | Synthetic peptide within Human APRIL/TNFSF13 aa 150-250. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC |
| WB:1:500-1:1,000: ICC:1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | A proliferation inducing ligand antibody
A proliferation-inducing ligand antibody
APRIL antibody
CD256 antibody
TALL-2 antibody
TALL2 antibody
TNF related death ligand antibody
TNF- and APOL-related leukocyte expressed ligand 2 antibody
TNF-related death ligand 1 antibody
TNF13_HUMAN antibody
TNFSF13 antibody
TRDL-1 antibody
TRDL1 antibody
Tumor necrosis factor (ligand) superfamily member 13 antibody Tumor necrosis factor ligand superfamily member 13 antibody Tumor necrosis factor like protein ZTNF2 antibody Tumor necrosis factor related death ligand 1 antibody UNQ383/PRO715 antibody ZTNF2 antibody |
|---|---|
| Molecular Weight(MW) | 16 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | O75888 |
|---|
Application
Fig1: Western blot analysis of APRIL/TNFSF13 on Hela cell and A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining of APRIL/TNFSF13 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-89, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).| Positive Control | Hela, A549. |
|---|---|
| Application Notes | WB:1:500-1:1,000: ICC:1:50: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
