Application
Fig1: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti- Collagen III (ER1906-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 130 kDApplication
Fig2: Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Collagen III Polyclonal Antibody, Unconjugated(ER1906-50) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | Collagen III |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COL 3A1 COL3A1 Collagen alpha 1(III) chain Collagen III alpha 1 chain precursor Collagen III alpha 1 polypeptide Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) Collagen type III alpha 1 Collagen type III alpha EDS4A Ehlers Danlos syndrome type IV, autosomal dominant Fetal collagen Type III collagen CO3A1_HUMAN Collagen alpha-1(III) chain Type III collagen type III preprocollagen alpha 1 chain.. |
| Product description | Type III Collagen is a homotrimer, or a protein composed of three identical peptide chains (monomers), each called an alpha 1 chain of type III collagen. Formally, the monomers are called collagen type III, alpha-1 chain and in humans are encoded by the COL3A1 gene. Type III collagen is one of the fibrillar collagens whose proteins have a long, inflexible, triple-helical domain. In addition to being an integral structural component of many organs, type III collagen is also an important regulator of the diameter of type I and II collagen fibrils. Type III collagen is also known to facilitate platelet aggregation through its binding to platelets and therefore, play an important role in blood clotting. Type III collagen is found as a major structural component in hollow organs such as large blood vessels, uterus and bowel. It is also found in many other tissues together with type I collagen. |
| Immunogen | KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain 801-900/1466 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRatCow |
| Concentration | 1mg/ml |
| Alternative Names | COL 3A1
COL3A1
Collagen alpha 1(III) chain
Collagen III alpha 1 chain precursor
Collagen III alpha 1 polypeptide
Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant)
Collagen type III alpha 1
Collagen type III alpha
EDS4A
Ehlers Danlos syndrome type IV autosomal dominant Fetal collagen Type III collagen CO3A1_HUMAN Collagen alpha-1(III) chain Type III collagen type III preprocollagen alpha 1 chain.. |
|---|---|
| Molecular Weight(MW) | 117 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | P02461 |
|---|
Application
Fig1: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti- Collagen III (ER1906-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 130 kD
Application
Fig2: Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Collagen III Polyclonal Antibody, Unconjugated(ER1906-50) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Hela Cell, human colon cancer. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
