Application
Fig1: Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Complement component C9a antibody (ER1906-54) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Raji Cell (Human) Lysate at 30 ug Primary: Anti- Complement component C9a (Bs- 11190R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26/61 kD Observed band size: 70 kD| Product Name | Complement component C9a |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C9a, C9 deficiency C9a C9 deficiency with dermatomyositis CO9_HUMAN Complement component 9 Complement component 9 deficiency Complement component C9a Complement component C9a C9. |
| Product description | Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC. |
| Immunogen | KLH conjugated synthetic peptide derived from human Complement component C9a 161-260/559 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | C9a C9 deficiency C9a C9 deficiency with dermatomyositis CO9_HUMAN Complement component 9 Complement component 9 deficiency Complement component C9a Complement component C9a C9. |
|---|---|
| Molecular Weight(MW) | 28/61 KDa |
| Cellular Localization | Secreted. Cell membrane; Multi-pass membrane protein. Note=Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore. |
| SwissProt ID | P02748 |
|---|
Application
Fig1: Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Complement component C9a antibody (ER1906-54) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Raji Cell (Human) Lysate at 30 ug Primary: Anti- Complement component C9a (Bs- 11190R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26/61 kD Observed band size: 70 kD| Positive Control | Plasma. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
