Application
Fig1: Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- Connexin-40 antibody(ER1906-57), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Antibody (ER1906-57, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of ER1906-57 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Connexin-40 Polyclonal Antibody, Unconjugated(ER1906-57) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Connexin-40 (ER1906-57) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD| Product Name | Connexin-40 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | connexin 40 connexin40 CX40 Gap junction alpha 5 protein gap junction protein alpha 5 40kD (connexin 40) gap junction protein alpha 5 GJA5 MGC11185 CXA5_HUMAN Gap junction alpha-5 protein Connexin-40 Cx40. |
| Product description | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
| Immunogen | KLH conjugated synthetic peptide derived from human Connexin-40 301-358/358 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | connexin 40 connexin40 CX40 Gap junction alpha 5 protein gap junction protein alpha 5 40kD (connexin 40) gap junction protein alpha 5 GJA5 MGC11185 CXA5_HUMAN Gap junction alpha-5 protein Connexin-40 Cx40. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. |
| SwissProt ID | P36382 |
|---|
Application
Fig1: Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- Connexin-40 antibody(ER1906-57), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Antibody (ER1906-57, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of ER1906-57 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Connexin-40 Polyclonal Antibody, Unconjugated(ER1906-57) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Connexin-40 (ER1906-57) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD| Positive Control | Rat myocardium tissue, human lung carcinoma, Nouse Heart Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
