IHC
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary for 20 minutes and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary for 20 minutes and DAB staining.WB
Sample:RSC96(Rat) Cell Lysate at 30 ug Primary: Anti- CPLX1 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 17 kDIF-P
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37℃, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei.IF-P
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37℃, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei.| Product Name | CPLX1 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Positively regulates a late step in synaptic vesicle exocytosis. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human CPLX1 31-100/134 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC-P:1:100-1:500 IF-P:1:100-1:500 |
|
| Species Reactivity | ChickenCowHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | complexin 1 Complexin I Complexin-1 CPLX1 CPLX1_HUMAN CPX I CPX-I CPX1 Synaphin 2 Synaphin-2 921-S. |
|---|---|
| Molecular Weight(MW) | 15 KDa |
| Cellular Localization | Cytoplasm; cytosol. Enriched at synaptic-releasing sites in mature neurons. |
| SwissProt ID | O14810 |
|---|
IHC
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary for 20 minutes and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary for 20 minutes and DAB staining.
WB
Sample:RSC96(Rat) Cell Lysate at 30 ug Primary: Anti- CPLX1 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 17 kD
IF-P
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37℃, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei.
IF-P
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37℃, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei.| Positive Control | Nervous system. In hippocampus and cerebellum, expressed mainly by inhibitory neurons. Overexpressed in substantia nigra from patients with Parkinson disease. |
|---|---|
| Application Notes | WB:1:500-1:2000 IHC-P:1:100-1:500 IF-P:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
