Application
Fig1: Western blot analysis of ABAT/GABA-T on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human liver tissue lysate Lane 2: Rat liver tissue lysateApplication
Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABAT/GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ABAT/GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ABAT/GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ABAT/GABA-T [JE50-16] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | (S) 3 amino 2 methylpropionate transaminase antibody (S)-3-amino-2-methylpropionate transaminase antibody 4 aminobutyrate aminotransferase antibody 4 aminobutyrate aminotransferase, mitochondrial antibody 4-aminobutyrate aminotransferase antibody ABAT antibody FLJ17813 antibody FLJ30272 antibody GABA aminotransferase antibody GABA AT antibody GABA T antibody GABA transaminase antibody GABA transferase antibody GABA-AT antibody GABA-T antibody GABAT antibody GABT_HUMAN antibody Gamma amino N butyrate transaminase antibody Gamma-amino-N-butyrate transaminase antibody hCG1984265 antibody L AIBAT antibody L-AIBAT antibody LAIBAT antibody mitochondrial antibody NPD009 antibody |
| Product description | 4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. |
| Immunogen | Synthetic peptide within N-terminal human ABAT/GABA-T. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500-1:1,000: IP:1:50: IHC:1:50-1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | (S) 3 amino 2 methylpropionate transaminase antibody
(S)-3-amino-2-methylpropionate transaminase antibody
4 aminobutyrate aminotransferase antibody
4 aminobutyrate aminotransferase mitochondrial antibody 4-aminobutyrate aminotransferase antibody ABAT antibody FLJ17813 antibody FLJ30272 antibody GABA aminotransferase antibody GABA AT antibody GABA T antibody GABA transaminase antibody GABA transferase antibody GABA-AT antibody GABA-T antibody GABAT antibody GABT_HUMAN antibody Gamma amino N butyrate transaminase antibody Gamma-amino-N-butyrate transaminase antibody hCG1984265 antibody L AIBAT antibody L-AIBAT antibody LAIBAT antibody mitochondrial antibody NPD009 antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Mitochondrion. |
Application
Fig1: Western blot analysis of ABAT/GABA-T on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human liver tissue lysate Lane 2: Rat liver tissue lysate
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABAT/GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ABAT/GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ABAT/GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Human liver tissue, rat liver tissue, human liver cancer tissue, human placenta tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000: IP:1:50: IHC:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
