Application
Fig1: Sample: heart (mouse) Lysate at 40 ug Primary: Anti-CRHR1(ER1906-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 58 kDApplication
Fig2: Sample: kidney (mouse) Lysate at 40 ug Primary: Anti-CRHR1(ER1906-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 58 kDApplication
Fig3: Sample: muscle (mouse) Lysate at 40 ug Primary: Anti-CRHR1(ER1906-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 58 kD| Product Name | CRHR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRF1 Corticotropin releasing hormone receptor variant 1h CRF-RI CRF 1 CRF R CRFR1 CRH R1h CRF-R1 CRHR 1 CRHR CRHR1f Releasing hormone receptor 1 Seven transmembrane helix receptor corticotropin releasing hormone receptor 1 Crhr MGC124237 MGC124240 CRF-R CRF-R-1 CRF-R1 CRFR-1 CRH-R-1 CRH-R1 CRH-R1h CRHR CRHR1L CRFR1_HUMAN. |
| Product description | Receptor for corticotropin releasing factor (CRH). Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. |
| Immunogen | KLH conjugated synthetic peptide derived from human CRHR1 55-150/444 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CRF1 Corticotropin releasing hormone receptor variant 1h CRF-RI CRF 1 CRF R CRFR1 CRH R1h CRF-R1 CRHR 1 CRHR CRHR1f Releasing hormone receptor 1 Seven transmembrane helix receptor corticotropin releasing hormone receptor 1 Crhr MGC124237 MGC124240 CRF-R CRF-R-1 CRF-R1 CRFR-1 CRH-R-1 CRH-R1 CRH-R1h CRHR CRHR1L CRFR1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P34998 |
|---|
Application
Fig1: Sample: heart (mouse) Lysate at 40 ug Primary: Anti-CRHR1(ER1906-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 58 kD
Application
Fig2: Sample: kidney (mouse) Lysate at 40 ug Primary: Anti-CRHR1(ER1906-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 58 kD
Application
Fig3: Sample: muscle (mouse) Lysate at 40 ug Primary: Anti-CRHR1(ER1906-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 58 kD| Positive Control | Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
