Application
Fig1: Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-CROT (ER1906-73) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kDApplication
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CROT Polyclonal Antibody, Unconjugated(ER1906-73) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | CROT |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carnitine O octanoyltransferase COT CROT OCTC_HUMAN Peroxisomal carnitine acyltransferase Peroxisomal carnitine O octanoyltransferase Peroxisomal carnitine O-octanoyltransferase. |
| Product description | Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. |
| Immunogen | KLH conjugated synthetic peptide derived from human CROT 41-140/612 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Carnitine O octanoyltransferase COT CROT OCTC_HUMAN Peroxisomal carnitine acyltransferase Peroxisomal carnitine O octanoyltransferase Peroxisomal carnitine O-octanoyltransferase. |
|---|---|
| Molecular Weight(MW) | 70 KDa |
| Cellular Localization | Peroxisome. |
| SwissProt ID | Q9UKG9 |
|---|
Application
Fig1: Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-CROT (ER1906-73) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD
Application
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CROT Polyclonal Antibody, Unconjugated(ER1906-73) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
