Application
Fig1: Sample: Hek293D serum Lysate at 40 ug Primary: Anti-CSEN (ER1906-75) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CSEN) Polyclonal Antibody, Unconjugated (ER1906-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CSEN) Polyclonal Antibody, Unconjugated (ER1906-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | CSEN |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A type potassium channel modulatory protein 3 A-type potassium channel modulatory protein 3 Calsenilin CSEN CSEN_HUMAN DRE antagonist modulator DRE-antagonist modulator DREAM EF hand transcription factor KCHIP 3 KCHIP3 KCNIP 3 KCNIP3 Kv channel interacting protein 3 Kv channel-interacting protein 3 MGC18289 Potassium channel interacting protein 3 Presenilin binding protein R74849. |
| Product description | Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity). ;Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and probably KCND3 trafficking to the cell surface. ;May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation. |
| Immunogen | KLH conjugated synthetic peptide derived from human CSEN 51-150/256 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | A type potassium channel modulatory protein 3 A-type potassium channel modulatory protein 3 Calsenilin CSEN CSEN_HUMAN DRE antagonist modulator DRE-antagonist modulator DREAM EF hand transcription factor KCHIP 3 KCHIP3 KCNIP 3 KCNIP3 Kv channel interacting protein 3 Kv channel-interacting protein 3 MGC18289 Potassium channel interacting protein 3 Presenilin binding protein R74849. |
|---|---|
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Cytoplasm. Cell membrane; Lipid-anchor. Endoplasmic reticulum. Golgi apparatus. Nucleus (Probable). Note=Also membrane-bound, associated with the plasma membrane. In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. |
| SwissProt ID | Q9Y2W7 |
|---|
Application
Fig1: Sample: Hek293D serum Lysate at 40 ug Primary: Anti-CSEN (ER1906-75) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CSEN) Polyclonal Antibody, Unconjugated (ER1906-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CSEN) Polyclonal Antibody, Unconjugated (ER1906-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
