Application
Fig1: Western blot analysis of NEK6 on THP-1 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | NEK6 [JE51-72] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Highly similar to cell cycle protein kinase CDC5/MSD2 antibody NEK6 antibody NEK6_HUMAN antibody Never in mitosis A-related kinase 6 antibody NIMA (Never In Mitosis Gene A) Related Kinase 6 antibody NimA related protein kinase 6 antibody NimA-related protein kinase 6 antibody Protein kinase SID6 1512 antibody Protein kinase SID6-1512 antibody Putative serine threonine protein kinase antibody Serine/threonine protein kinase Nek6 antibody Serine/threonine-protein kinase Nek6 antibody SID6 1512 antibody SID61512 antibody |
| Product description | Serine/threonine-protein kinase Nek6 is an enzyme that in humans is encoded by the NEK6 gene. The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. It is a protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. |
| Immunogen | Recombinant protein within N-terminal human NEK6 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
NEK6 antibody
NEK6_HUMAN antibody Never in mitosis A-related kinase 6 antibody NIMA (Never In Mitosis Gene A) Related Kinase 6 antibody NimA related protein kinase 6 antibody NimA-related protein kinase 6 antibody Protein kinase SID6 1512 antibody Protein kinase SID6-1512 antibody Putative serine threonine protein kinase antibody Serine/threonine protein kinase Nek6 antibody Serine/threonine-protein kinase Nek6 antibody SID6 1512 antibody SID61512 antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa |
| Cellular Localization | Nucleus, nucleus speckle, centrosome, spindle pole, cytoplasm. |
| SwissProt ID | Q9HC98 |
|---|
Application
Fig1: Western blot analysis of NEK6 on THP-1 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | THP-1 cell. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
