Application
Fig1: Sample: Testis (Rat) Lysate at 40 ug Primary: Anti-CT054 (ER1906-78) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kDApplication
Fig2: Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-CT054 antibody (ER1906-78) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | CT054 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bA371L19.1 BVVLS C20orf54 C20orf54provided by HGNC Chromosome 20 open reading frame 54 hRFT2 MGC10698 S52A3_HUMAN RFT2 Riboflavin transporter 2. |
| Product description | Riboflavin transporter. Riboflavin transport is Na(+)-independent but moderately pH-sensitive. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), and to a lesser extent by amiloride. |
| Immunogen | KLH conjugated synthetic peptide derived from human CT054/C20orf54 391-469/469 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | bA371L19.1 BVVLS C20orf54 C20orf54provided by HGNC Chromosome 20 open reading frame 54 hRFT2 MGC10698 S52A3_HUMAN RFT2 Riboflavin transporter 2. |
|---|---|
| Molecular Weight(MW) | 51 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q9NQ40 |
|---|
Application
Fig1: Sample: Testis (Rat) Lysate at 40 ug Primary: Anti-CT054 (ER1906-78) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Application
Fig2: Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-CT054 antibody (ER1906-78) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Predominantly expressed in testis. Highly expressed in small intestine and prostate. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
