Application
Fig1: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 35 kDApplication
Fig2: Sample: Liver(Mouse) Cell Lysate at 30 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 35 kDApplication
Fig3: Sample: Liver(Rat) Cell Lysate at 30 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 35 kDApplication
Fig4: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kDApplication
Fig5: Blank control: Raji(blue). Primary Antibody: Rabbit Anti-CTLA4 antibody(ER1906-81), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig6: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kDApplication
Fig7: Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).Application
Fig8: Blank control(blue):Mouse Spleen Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti- Phospho-c-Fos (Thr325)/FITC Conjugated antibody (ER1906-81 /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.Application
Fig9: Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min) ). Primary Antibody: Rabbit Anti-CTLA4/FITC Conjugated antibody (ER1906-81/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC orange) ,used under the same conditions.| Product Name | CTLA4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 152 CD152 CD152 antigen Celiac disease 3 CELIAC3 CTLA 4 CTLA-4 Cytotoxic T cell associated 4 Cytotoxic T lymphocyte associated 4 Cytotoxic T lymphocyte associated antigen 4 Cytotoxic T lymphocyte associated protein 4 Cytotoxic T lymphocyte associated serine esterase 4 Cytotoxic T lymphocyte protein 4 Cytotoxic T-lymphocyte-associated antigen 4 Cytotoxic T-lymphocyte-associated protein 4 GSE IDDM12. |
| Product description | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Immunogen | KLH conjugated synthetic peptide derived from human CTLA-4/CD152 75-170/223 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | CD 152 CD152 CD152 antigen Celiac disease 3 CELIAC3 CTLA 4 CTLA-4 Cytotoxic T cell associated 4 Cytotoxic T lymphocyte associated 4 Cytotoxic T lymphocyte associated antigen 4 Cytotoxic T lymphocyte associated protein 4 Cytotoxic T lymphocyte associated serine esterase 4 Cytotoxic T lymphocyte protein 4 Cytotoxic T-lymphocyte-associated antigen 4 Cytotoxic T-lymphocyte-associated protein 4 GSE IDDM12. |
|---|---|
| Molecular Weight(MW) | 21 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation. |
| SwissProt ID | P16410 |
|---|
Application
Fig1: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 35 kD
Application
Fig2: Sample: Liver(Mouse) Cell Lysate at 30 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 35 kD
Application
Fig3: Sample: Liver(Rat) Cell Lysate at 30 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 35 kD
Application
Fig4: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD
Application
Fig5: Blank control: Raji(blue). Primary Antibody: Rabbit Anti-CTLA4 antibody(ER1906-81), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig6: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-CTLA4 (ER1906-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD
Application
Fig7: Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
Application
Fig8: Blank control(blue):Mouse Spleen Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti- Phospho-c-Fos (Thr325)/FITC Conjugated antibody (ER1906-81 /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Application
Fig9: Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min) ). Primary Antibody: Rabbit Anti-CTLA4/FITC Conjugated antibody (ER1906-81/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC orange) ,used under the same conditions.| Positive Control | Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
