Application
Fig1: Sample: MDA-MB-231(Human) Cell Lysate at 30 ug Primary: Anti-CWH43 (ER1906-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 79 kD Observed band size: 75 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CWH43) Polyclonal Antibody, Unconjugated (ER1906-83) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CWH43) Polyclonal Antibody, Unconjugated (ER1906-83) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | CWH43 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cell wall biogenesis protein 43 C-terminal homolog Cwh43 imds 60 PG2IP_HUMAN PGAP2-interacting protein. |
| Product description | Involved in lipid remodeling during GPI-anchor maturation (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human CWH43 501-600/699 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Cell wall biogenesis protein 43 C-terminal homolog Cwh43 imds 60 PG2IP_HUMAN PGAP2-interacting protein. |
|---|---|
| Molecular Weight(MW) | 79 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein (Potential). |
| SwissProt ID | Q9H720 |
|---|
Application
Fig1: Sample: MDA-MB-231(Human) Cell Lysate at 30 ug Primary: Anti-CWH43 (ER1906-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 79 kD Observed band size: 75 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CWH43) Polyclonal Antibody, Unconjugated (ER1906-83) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CWH43) Polyclonal Antibody, Unconjugated (ER1906-83) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
