Application
Fig1: Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-CX3CR1 (ER1906-84) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39/44 kDApplication
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-CX3CR1 (ER1906-84) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kDApplication
Fig3: Sample:Lung(Mouse) lysate at 30ug; Primary: Anti-CX3CR1 (ER1906-84) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 39kD Observed band size : 39kD| Product Name | CX3CR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Beta chemokine receptor-like 1 C X3 C CKR 1 CCRL1 Chemokine C X3 C motif receptor 1 CMK BRL 1 CMKBLR1 CMK-BRL-1 CMKDR1 CX3C chemokine receptor 1 CX3C CKR1 Fractalkine Receptor GPR13 GPRV28 V28 Chemokine receptor 1(chemokine (C-X3-C motif) receptor 1 C-X3-C CKR-1 CX3C1_HUMAN CX3CR1 G-protein coupled receptor 13. |
| Product description | Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. |
| Immunogen | KLH conjugated synthetic peptide derived from human CX3CR1 151-250/355 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Beta chemokine receptor-like 1 C X3 C CKR 1 CCRL1 Chemokine C X3 C motif receptor 1 CMK BRL 1 CMKBLR1 CMK-BRL-1 CMKDR1 CX3C chemokine receptor 1 CX3C CKR1 Fractalkine Receptor GPR13 GPRV28 V28 Chemokine receptor 1(chemokine (C-X3-C motif) receptor 1 C-X3-C CKR-1 CX3C1_HUMAN CX3CR1 G-protein coupled receptor 13. |
|---|---|
| Molecular Weight(MW) | 39 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P49238 |
|---|
Application
Fig1: Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-CX3CR1 (ER1906-84) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39/44 kD
Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-CX3CR1 (ER1906-84) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD
Application
Fig3: Sample:Lung(Mouse) lysate at 30ug; Primary: Anti-CX3CR1 (ER1906-84) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 39kD Observed band size : 39kD| Positive Control | Expressed in lymphoid and neural tissues. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
