Application
Fig1: Sample: BV2 Cell (Mouse) Lysate at 40 ug Primary: Anti- CXCL16 (ER1906-85) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kDApplication
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-CXCL16 antibody (ER1906-85) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig3: Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (ER1906-85) at 1:50 for two hours at room temperature (green channel).| Product Name | CXCL16 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CXC-chemokine ligand 16 Chemokine (C X C motif) ligand 16 CXC chemokine ligand 16 CXCLG16 Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein SCYB16 Small inducible cytokine B16 precursor SRPSOX Transmembrane chemokine CXCL16 CXL16_HUMAN. |
| Product description | Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17. |
| Immunogen | KLH conjugated synthetic peptide derived from the middle of human CXCL16 85-200/247 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IFFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CXC-chemokine ligand 16 Chemokine (C X C motif) ligand 16 CXC chemokine ligand 16 CXCLG16 Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein SCYB16 Small inducible cytokine B16 precursor SRPSOX Transmembrane chemokine CXCL16 CXL16_HUMAN. |
|---|---|
| Molecular Weight(MW) | 27 KDa |
| Cellular Localization | Cell membrane. Secreted. Also exists as a soluble form. |
| SwissProt ID | Q8BSU2 |
|---|
Application
Fig1: Sample: BV2 Cell (Mouse) Lysate at 40 ug Primary: Anti- CXCL16 (ER1906-85) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD
Application
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-CXCL16 antibody (ER1906-85) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig3: Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (ER1906-85) at 1:50 for two hours at room temperature (green channel).| Positive Control | Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: IF:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
