Application
Fig1: Sample: liver(mouse) Lysate at 40 ug Primary: Anti-CXCR3 (ER1906-88)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41kD Observed band size: 51kDApplication
Fig2: Sample: heart(mouse) Lysate at 40 ug Primary: Anti-CXCR3 (ER1906-88)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41kD Observed band size: 51kDApplication
Fig3: Blank control: HCCLM3(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5ul in 100 uL1X PBS containing 0.5% BSA(green).Application
Fig4: Blank control: U937. Primary Antibody (green line): Rabbit Anti-CXCR3 antibody (ER1906-88) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | CXCR3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CXC-R3 Interferon-inducible protein 10 receptor IP-10 receptor CKR-L2 CD183 antigen G protein-coupled receptor 9 C-X-C Chemokine receptor CD183 CXC Motif Receptor 3 CKRL2 CMKAR3 G Protein Coupled Receptor 9 GPR9 IP10 IP10R MIGR CXCR3_HUMAN. |
| Product description | Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). ;Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis. ;Isoform 3 mediates the activity of CXCL11. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CXCR3 301-367/367 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:0.2μg /test: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | CXC-R3 Interferon-inducible protein 10 receptor IP-10 receptor CKR-L2 CD183 antigen G protein-coupled receptor 9 C-X-C Chemokine receptor CD183 CXC Motif Receptor 3 CKRL2 CMKAR3 G Protein Coupled Receptor 9 GPR9 IP10 IP10R MIGR CXCR3_HUMAN. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | O88410 |
|---|
Application
Fig1: Sample: liver(mouse) Lysate at 40 ug Primary: Anti-CXCR3 (ER1906-88)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41kD Observed band size: 51kD
Application
Fig2: Sample: heart(mouse) Lysate at 40 ug Primary: Anti-CXCR3 (ER1906-88)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41kD Observed band size: 51kD
Application
Fig3: Blank control: HCCLM3(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5ul in 100 uL1X PBS containing 0.5% BSA(green).
Application
Fig4: Blank control: U937. Primary Antibody (green line): Rabbit Anti-CXCR3 antibody (ER1906-88) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. |
|---|---|
| Application Notes | WB:1:500-2000: FC:0.2μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
