Application
Fig1: Blank control (blue line): Mouse Spleen cells (fixed with 70% methanol (Overnight at 4℃)). Primary Antibody (green line): Rabbit Anti-CXCR4 antibody (ER1906-89),Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITCApplication
Fig2: Sample:liver(mouse) Lysate at 40 ug Primary: Anti-CXCR4(ER1906-89)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40kD Observed band size: 40kD| Product Name | CXCR4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C-X-C chemokine receptor type 4 CXC-R4 CXCR-4 Stromal cell-derived factor 1 receptor SDF-1 receptor Fusin Leukocyte-derived seven transmembrane domain receptor LESTR CD184 antigen CXCR4_HUMAN. |
| Product description | Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CXCR4 151-300/359 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3μg/Test: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Alternative Names | C-X-C chemokine receptor type 4 CXC-R4 CXCR-4 Stromal cell-derived factor 1 receptor SDF-1 receptor Fusin Leukocyte-derived seven transmembrane domain receptor LESTR CD184 antigen CXCR4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P70658 |
|---|
Application
Fig1: Blank control (blue line): Mouse Spleen cells (fixed with 70% methanol (Overnight at 4℃)). Primary Antibody (green line): Rabbit Anti-CXCR4 antibody (ER1906-89),Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Application
Fig2: Sample:liver(mouse) Lysate at 40 ug Primary: Anti-CXCR4(ER1906-89)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40kD Observed band size: 40kD| Positive Control | Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
