Application
Fig1: Sample: Blood(Mouse) Cell Lysate at 30 ug Primary: Anti-CYB5R3 (ER1906-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kDApplication
Fig2: Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti- CYB5R3 (ER1906-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD| Product Name | CYB5R3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B5R Cyb5r3 Cytochrome b5 reductase 3 Cytochrome b5 reductase DIA1 Diaphorase 1 Diaphorase-1 NADH cytochrome b5 reductase 3 NADH-cytochrome b5 reductase 3 membrane-bound form NADH-cytochrome b5 reductase 3 soluble form NB5R3_HUMAN OTTHUMP00000028761 OTTHUMP00000198435 OTTHUMP00000198574 OTTHUMP00000198662 OTTHUMP00000198665. |
| Product description | Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. |
| Immunogen | KLH conjugated synthetic peptide derived from human CYB5R3 101-200/301 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | B5R Cyb5r3 Cytochrome b5 reductase 3 Cytochrome b5 reductase DIA1 Diaphorase 1 Diaphorase-1 NADH cytochrome b5 reductase 3 NADH-cytochrome b5 reductase 3 membrane-bound form NADH-cytochrome b5 reductase 3 soluble form NB5R3_HUMAN OTTHUMP00000028761 OTTHUMP00000198435 OTTHUMP00000198574 OTTHUMP00000198662 OTTHUMP00000198665. |
|---|---|
| Molecular Weight(MW) | 34 KDa |
| Cellular Localization | Endoplasmic reticulum membrane. Mitochondrion outer membrane and Cytoplasm. Produces the soluble form found in erythrocytes. |
| SwissProt ID | P00387 |
|---|
Application
Fig1: Sample: Blood(Mouse) Cell Lysate at 30 ug Primary: Anti-CYB5R3 (ER1906-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD
Application
Fig2: Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti- CYB5R3 (ER1906-92) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD| Positive Control | Isoform 2 is expressed at late stages of erythroid maturation. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
