Application
Fig1: Sample: U-2OS(Human) Cell Lysate at 30 ug Primary: Anti- Cyclin F (ER1906-95) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD| Product Name | Cyclin F |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCNF F box only protein 1 FBX1 FBXO1 G2 mitotic specific cyclin F CCNF_HUMAN. |
| Product description | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. |
| Immunogen | KLH conjugated synthetic peptide derived from human Cyclin F 451-550/786 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CCNF F box only protein 1 FBX1 FBXO1 G2 mitotic specific cyclin F CCNF_HUMAN. |
|---|---|
| Molecular Weight(MW) | 86 KDa |
| Cellular Localization | Nucleus. Cytoplasm, cytoskeleton, centrosome, centriole. Note=Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Also localizes to the nucleus. |
| SwissProt ID | P41002 |
|---|
Application
Fig1: Sample: U-2OS(Human) Cell Lysate at 30 ug Primary: Anti- Cyclin F (ER1906-95) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kD Observed band size: 86 kD| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
