Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CYLD) Polyclonal Antibody, Unconjugated (ER1906-97) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-CYLD (ER1906-97) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kD Observed band size: 105 kD| Product Name | CYLD |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDMT cylindromatosis (turban tumor syndrome) cylindromatosis 1 Deubiquitinating enzyme CYLD EAC HSPC057 KIAA0849 turban tumor syndrome Ubiquitin thiolesterase CYLD Ubiquitin-specific processing protease CYLD CYLD_HUMAN Ubiquitin carboxyl-terminal hydrolase CYLD CYLD BRSS CDMT CYLD1 CYLDI EAC MFT MFT1 SBS TEM USPL2. |
| Product description | Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis |
| Immunogen | KLH conjugated synthetic peptide derived from human cylindromatosis 1 501-600/956 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | CDMT cylindromatosis (turban tumor syndrome) cylindromatosis 1 Deubiquitinating enzyme CYLD EAC HSPC057 KIAA0849 turban tumor syndrome Ubiquitin thiolesterase CYLD Ubiquitin-specific processing protease CYLD CYLD_HUMAN Ubiquitin carboxyl-terminal hydrolase CYLD CYLD BRSS CDMT CYLD1 CYLDI EAC MFT MFT1 SBS TEM USPL2. |
|---|---|
| Molecular Weight(MW) | 105 KDa |
| Cellular Localization | Cytoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Detected at the microtubule cytoskeleton during interphase. Detected at the midbody during telophase. |
| SwissProt ID | Q9NQC7 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CYLD) Polyclonal Antibody, Unconjugated (ER1906-97) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-CYLD (ER1906-97) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kD Observed band size: 105 kD| Positive Control | Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
