Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (ER1906-98) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Lane 1: Brain(Rat) Lysate at 30 ug Lane 2: Kidney(Rat)Lysate at 30 ug Primary: Anti-CYP11A1/P450SCC (ER1906-98) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 53kD Observed band size : 53kDApplication
Fig3: Sample: Placenta (Mouse) Lysate at 30 ug Primary: Anti- CYP11A1 (Ser576) (ER1906-98) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 53/57 kD Observed band size: 57 kD| Product Name | CYP11A1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cholesterol 20 22 desmolase； Cholesterol desmolase； Cholesterol monooxygenase (side chain cleaving)； Cholesterol side chain cleavage enzyme； Cholesterol side chain cleavage enzyme mitochondrial； Cholesterol side-chain cleavage enzyme； CP11A_HUMAN； CYP11A； CYP11A1； CYPXIA1； Cytochrome P450 11A1； Cytochrome P450 11A1 mitochondrial； Cytochrome P450 family 11 subfamily A polypeptide 1； Cytochrome P450 subfamily XIA； Cytochrome P450(scc)； Cytochrome P450C11A1； mitochondrial； P450SCC； Steroid 20 22 lyase. |
| Product description | Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. |
| Immunogen | KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC 321-420/521 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cholesterol 20 22 desmolase； Cholesterol desmolase； Cholesterol monooxygenase (side chain cleaving)； Cholesterol side chain cleavage enzyme； Cholesterol side chain cleavage enzyme mitochondrial； Cholesterol side-chain cleavage enzyme； CP11A_HUMAN； CYP11A； CYP11A1； CYPXIA1； Cytochrome P450 11A1； Cytochrome P450 11A1 mitochondrial； Cytochrome P450 family 11 subfamily A polypeptide 1； Cytochrome P450 subfamily XIA； Cytochrome P450(scc)； Cytochrome P450C11A1； mitochondrial； P450SCC； Steroid 20 22 lyase. |
|---|---|
| Molecular Weight(MW) | 53/57 KDa |
| Cellular Localization | Mitochondrion membrane. |
| SwissProt ID | P05108 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (ER1906-98) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Lane 1: Brain(Rat) Lysate at 30 ug Lane 2: Kidney(Rat)Lysate at 30 ug Primary: Anti-CYP11A1/P450SCC (ER1906-98) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 53kD Observed band size : 53kD
Application
Fig3: Sample: Placenta (Mouse) Lysate at 30 ug Primary: Anti- CYP11A1 (Ser576) (ER1906-98) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 53/57 kD Observed band size: 57 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
