Application
Fig1: Sample: Kidney (Rat) Lysate at 40 ug Brain (Rat) Lysate at 40 ug Primary: Anti-CYP11B1 (ER1906-99) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CYP11B1) Polyclonal Antibody, Unconjugated (ER1906-99) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- CYP11B1 (ER1906-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Product Name | CYP11B1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CPN1 CYP11B CYPXIB1 Cytochrome P450 11B1, mitochondrial Cytochrome p450 XIB1 Cytochrome P450, family 11, subfamily B, polypeptide 1 FHI P 450C11 P450C11 S11BH Steroid 11 beta hydroxylase Steroid 11 beta monooxygenase C11B1_HUMAN Cytochrome P-450c11 Cytochrome P450C11 Steroid 11-beta-hydroxylase. |
| Product description | Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB. |
| Immunogen | KLH conjugated synthetic peptide derived from human CYP11B1 301-400/503 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | CPN1
CYP11B
CYPXIB1
Cytochrome P450 11B1 mitochondrial Cytochrome p450 XIB1 Cytochrome P450 family 11 subfamily B polypeptide 1 FHI P 450C11 P450C11 S11BH Steroid 11 beta hydroxylase Steroid 11 beta monooxygenase C11B1_HUMAN Cytochrome P-450c11 Cytochrome P450C11 Steroid 11-beta-hydroxylase. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| SwissProt ID | P15538 |
|---|
Application
Fig1: Sample: Kidney (Rat) Lysate at 40 ug Brain (Rat) Lysate at 40 ug Primary: Anti-CYP11B1 (ER1906-99) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CYP11B1) Polyclonal Antibody, Unconjugated (ER1906-99) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- CYP11B1 (ER1906-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Positive Control | Mitochondrion membrane. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
