Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CYP2C19) Polyclonal Antibody, Unconjugated (ER1907-04) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- CYP2C19 (ER1907-04) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD| Product Name | CYP2C19 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI266900 CYP2C CYP2C19 CYP2C37 CYP2C50 Cyp2c54 CYP450-2C CPCJ CYPIIC17 CYPIIC19 EG404195 LOC293989 MGC109053 P450-11A P450C2C P450IIC19 CP2CJ_HUMAN Cytochrome P450 2C19 R)-limonene 6-monooxygenase (S)-limonene 6-monooxygenase (S)-limonene 7-monooxygenase CYPIIC17 CYPIIC19 Cytochrome P450-11A Cytochrome P450-254C Mephenytoin 4-hydroxylase. |
| Product description | Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, |
| Immunogen | KLH conjugated synthetic peptide derived from human CYP2C19 201-300/490 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AI266900 CYP2C CYP2C19 CYP2C37 CYP2C50 Cyp2c54 CYP450-2C CPCJ CYPIIC17 CYPIIC19 EG404195 LOC293989 MGC109053 P450-11A P450C2C P450IIC19 CP2CJ_HUMAN Cytochrome P450 2C19 R)-limonene 6-monooxygenase (S)-limonene 6-monooxygenase (S)-limonene 7-monooxygenase CYPIIC17 CYPIIC19 Cytochrome P450-11A Cytochrome P450-254C Mephenytoin 4-hydroxylase. |
|---|---|
| Molecular Weight(MW) | 56 KDa |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| SwissProt ID | P33261 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CYP2C19) Polyclonal Antibody, Unconjugated (ER1907-04) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- CYP2C19 (ER1907-04) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
