Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CYP8B1) Polyclonal Antibody, Unconjugated (ER1907-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-CYP8B1 (ER1907-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Product Name | CYP8B1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase CP8B1_HUMAN CYP12 CYP8B1 CYPVIIIB1 Cytochrome P450 8B1 Cytochrome P450, family 8, subfamily B, polypeptide 1 Sterol 12-alpha-hydroxylase. |
| Product description | Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids. |
| Immunogen | KLH conjugated synthetic peptide derived from human CYP8B1 151-250/501 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
CP8B1_HUMAN
CYP12
CYP8B1
CYPVIIIB1
Cytochrome P450 8B1
Cytochrome P450 family 8 subfamily B polypeptide 1 Sterol 12-alpha-hydroxylase. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| Cellular Localization | Endoplasmic reticulum membrane. Microsome membrane. |
| SwissProt ID | Q9UNU6 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CYP8B1) Polyclonal Antibody, Unconjugated (ER1907-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-CYP8B1 (ER1907-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Positive Control | Liver. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
