Application
Fig1: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cytochrome B Antibody(ER1907-11)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Cytochrome B (ER1907-11) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD| Product Name | Cytochrome B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MT-CYB COB Complex III subunit 3 Complex III subunit III CYTB Cytochrome b c1 complex subunit 3 Mitochondrially encoded cytochrome b MTCYB Ubiquinol cytochrome c reductase complex cytochrome b subunit CYB_HUMAN. |
| Product description | Cytochrome b is a protein found in the mitochondria of eukaryotic cells. It functions as part of the electron transport chain and is the main subunit of transmembrane cytochrome bc1 and b6f complexes. In the mitochondrion of eukaryotes and in aerobic prokaryotes, cytochrome b is a component of respiratory chain complex III, also known as the bc1 complex or ubiquinol-cytochrome c reductase. In plant chloroplasts and cyanobacteria, there is an analogous protein, cytochrome b6, a component of the plastoquinone-plastocyanin reductase, also known as the b6f complex. These complexes are involved in electron transport, the pumping of protons to create a proton-motive force (PMF). This proton gradient is used for the generation of ATP. These complexes play a vital role in cells. Mutations in cytochrome b primarily result in exercise intolerance in human patients; though more rare, severe multi-system pathologies have also been reported. Single-point mutations in cytochrome b of Plasmodium falciparum and P. berghei are associated with resistance to the anti-malarial drug atovaquone. |
| Immunogen | KLH conjugated synthetic peptide derived from human Cytochrome B 101-200/388 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/test: |
|
| Species Reactivity | HumanMouseRatPig |
| Concentration | 1mg/ml |
| Alternative Names | MT-CYB
COB
Complex III subunit 3 Complex III subunit III CYTB Cytochrome b c1 complex subunit 3 Mitochondrially encoded cytochrome b MTCYB Ubiquinol cytochrome c reductase complex cytochrome b subunit CYB_HUMAN. |
|---|---|
| Molecular Weight(MW) | 43 KDa |
| Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein. |
| SwissProt ID | P00156 |
|---|
Application
Fig1: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cytochrome B Antibody(ER1907-11)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Cytochrome B (ER1907-11) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD| Positive Control | U-937 cells, Hela(Human) Cell Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
