Application
Fig1: Sample: Large intestine (Mouse) Lysate at 40 ug Large intestine(Rat) Lysate at 40 ug Primary: Anti- Cytokeratin 7 (ER1907-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kDApplication
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cytokeratin 7 Polyclonal Antibody, Unconjugated(ER1907-15) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Sample: Large intestine (Mouse) Lysate at 30 ug Primary: Anti- Cytokeratin 7 (ER1907-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kDApplication
Fig4: Sample: Placenta (Mouse) Lysate at 30 ug Lung (Mouse) Lysate at 30 ug Primary: Anti-Cytokeratin 7 (ER1907-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kDApplication
Fig5: Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti- Cytokeratin 7 (ER1907-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD| Product Name | Cytokeratin 7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CK 7 CK-7 ck7 Cytokeratin 7 Cytokeratin-7 Cytokeratin7 D15Wsu77e K2C7 K2C7_HUMAN K7 Keratin 55k type ii cytoskeletal Keratin 7 Keratin simple epithelial type 1 k7 Keratin type II cytoskeletal 7 Keratin type ii cytoskletal 7 Keratin, 55K type II cytoskeletal Keratin, simple epithelial Keratin, simple epithelial type I, K7 Keratin, type II cytoskeletal 7 Keratin-7 Keratin7 KRT 7 Krt2-7 KRT7 MGC11625 MGC129731 mgc3625 Sarcolectin SCL Type ii mesothelial keratin k7 Type-II keratin Kb7 |
| Product description | Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). |
| Immunogen | KLH conjugated synthetic peptide derived from the middle of mouse CK7 251-350/469 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CK 7
CK-7
ck7
Cytokeratin 7
Cytokeratin-7
Cytokeratin7
D15Wsu77e
K2C7
K2C7_HUMAN
K7
Keratin 55k type ii cytoskeletal
Keratin 7
Keratin simple epithelial type 1 k7
Keratin type II cytoskeletal 7
Keratin type ii cytoskletal 7
Keratin 55K type II cytoskeletal Keratin simple epithelial Keratin simple epithelial type I K7 Keratin type II cytoskeletal 7 Keratin-7 Keratin7 KRT 7 Krt2-7 KRT7 MGC11625 MGC129731 mgc3625 Sarcolectin SCL Type ii mesothelial keratin k7 Type-II keratin Kb7 |
|---|---|
| Molecular Weight(MW) | 54 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q9DCV7 |
|---|
Application
Fig1: Sample: Large intestine (Mouse) Lysate at 40 ug Large intestine(Rat) Lysate at 40 ug Primary: Anti- Cytokeratin 7 (ER1907-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD
Application
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cytokeratin 7 Polyclonal Antibody, Unconjugated(ER1907-15) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Sample: Large intestine (Mouse) Lysate at 30 ug Primary: Anti- Cytokeratin 7 (ER1907-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kD
Application
Fig4: Sample: Placenta (Mouse) Lysate at 30 ug Lung (Mouse) Lysate at 30 ug Primary: Anti-Cytokeratin 7 (ER1907-15) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kD
Application
Fig5: Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti- Cytokeratin 7 (ER1907-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD| Positive Control | Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
