Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (D4S234E) Polyclonal Antibody, Unconjugated (ER1907-16) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-D4S234E (ER1907-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kDApplication
Fig3: Sample: Cerebral cortex (Mouse) Lysate at 40 ug Primary: Anti-D4S234E (ER1907-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kDApplication
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-D4S234E (ER1907-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD| Product Name | D4S234E |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NSG1_HUMAN Brain neuron cytoplasmic protein 1 Brain specific mRNA b Bsmrb Neuron-specific gene family member 1 Neuron-specific protein family member 1 NSG1 D4S234 NEEP21 P21. |
| Immunogen | KLH conjugated synthetic peptide derived from human NEEP21 1-100/185 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | NSG1_HUMAN Brain neuron cytoplasmic protein 1 Brain specific mRNA b Bsmrb Neuron-specific gene family member 1 Neuron-specific protein family member 1 NSG1 D4S234 NEEP21 P21. |
|---|---|
| Molecular Weight(MW) | 21 KDa |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein (Potential). |
| SwissProt ID | P42857 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (D4S234E) Polyclonal Antibody, Unconjugated (ER1907-16) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-D4S234E (ER1907-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD
Application
Fig3: Sample: Cerebral cortex (Mouse) Lysate at 40 ug Primary: Anti-D4S234E (ER1907-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD
Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-D4S234E (ER1907-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
