Application
Fig1: Sample: Kidney(Mouse) Lysate at 30 ug HepG2 Cell Lysate at 30 ug Primary: Anti-DAPK2 (ER1907-18) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 dilution; Predicted band size :41 kD Observed band size : 41 kDApplication
Fig2: Sample:293T Cell Lysate at 30 ug Primary: Anti-DAPK2 (ER1907-18) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 dilution; Predicted band size :41 kD Observed band size : 41 kD| Product Name | DAPK2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Death associated protein kinase 2 DAK 2 DAK2 DAP kinase related protein 1 DAPK 2 DAPK2 Death associated protein kinase 2 DRP 1 DRP1 DRP-1 MGC119312 DAP kinase 2 DAP kinase related protein 1 DAP kinase related protein 1 beta isoform DAP-kinase-related protein 1 DAPK 2 DAPK2 DAPK2_HUMAN Death associated protein kinase 2 Death-associated protein kinase 2 DRP 1 DRP-1 DRP1 MGC119312. |
| Product description | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation.;Isoform 2 can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death. |
| Immunogen | KLH conjugated synthetic peptide derived from human DAPK2 271-370/370 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDog |
| Concentration | 1mg/ml |
| Alternative Names | Death associated protein kinase 2 DAK 2 DAK2 DAP kinase related protein 1 DAPK 2 DAPK2 Death associated protein kinase 2 DRP 1 DRP1 DRP-1 MGC119312 DAP kinase 2 DAP kinase related protein 1 DAP kinase related protein 1 beta isoform DAP-kinase-related protein 1 DAPK 2 DAPK2 DAPK2_HUMAN Death associated protein kinase 2 Death-associated protein kinase 2 DRP 1 DRP-1 DRP1 MGC119312. |
|---|---|
| Molecular Weight(MW) | 41 KDa |
| Cellular Localization | Cytoplasm. Cytoplasmic vesicle, autophagosome lumen. |
| SwissProt ID | Q9UIK4 |
|---|
Application
Fig1: Sample: Kidney(Mouse) Lysate at 30 ug HepG2 Cell Lysate at 30 ug Primary: Anti-DAPK2 (ER1907-18) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 dilution; Predicted band size :41 kD Observed band size : 41 kD
Application
Fig2: Sample:293T Cell Lysate at 30 ug Primary: Anti-DAPK2 (ER1907-18) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 dilution; Predicted band size :41 kD Observed band size : 41 kD| Positive Control | Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. It is expressed abundantly in cells differentiated toward granulocytes and low in undifferentiated, normal and leukemic hematopoietic cells and monocytes/macrophages. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
