Application
Fig1: Western blot analysis of ECM1 on rat heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-41, 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining of ECM1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-41, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).Application
Fig3: ICC staining of ECM1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-41, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-ECM1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-41, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ECM1 [JE54-17] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ECM 1 antibody Ecm1 antibody ECM1_HUMAN antibody Extracellular matrix protein 1 antibody Secretory component p85 antibody URBWD antibody |
| Product description | ECM1 (extracellular matrix protein 1), also known as secretory component p85, is a secreted glycoprotein that is essential for the proper structure and function of the skin. It is widely expressed and localizes to the extracellular matrix. ECM1 binds to a variety of extracellular matrix components, including Perlecan, Fibulin and matrix metalloproteinase-9 (MMP-9), and participates in the structural organization of the dermis. In addition, ECM1 enhances the association of Collagen Type IV with Laminin 332 suggesting that it is a key player in interstitial dermis and the dermal-epidermal junction. Mutations in the gene encoding ECM1 result in the autosomal recessive disorder lipoid proteinosis (LiP). LiP is characterized by hyalinization of the dermis and reduplication of the basement membrane of the skin. LiP patients exhibit thickening of the skin and mucosae. Four splice variants (known as ECM1a-ECM1d) exist for ECM1. |
| Immunogen | Synthetic peptide within N terminal human ECM1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHC-P |
| WB:1:1,000-1:5,000: ICC:1:50-1:100: IHC-P:1:50-1:200: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | ECM 1 antibody
Ecm1 antibody
ECM1_HUMAN antibody Extracellular matrix protein 1 antibody Secretory component p85 antibody URBWD antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | extracellular matrix |
Application
Fig1: Western blot analysis of ECM1 on rat heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-41, 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining of ECM1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-41, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig3: ICC staining of ECM1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7110-41, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-ECM1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-41, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Rat heart tissue lysates, MCF-7, SiHa, human esophagus tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000: ICC:1:50-1:100: IHC-P:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
