Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti-DBF4B (ER1907-21) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18kD Observed band size: 18kDApplication
Fig2: Sample: Hela Cell (Human) Lysate at 30 ug Primary: Anti-DBF4B (ER1907-21) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18kD Observed band size: 18kDApplication
Fig3: Sample: U2os Cell (Human) Lysate at 30 ug Primary: Anti-DBF4B (ER1907-21) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18kD Observed band size: 18kD| Product Name | DBF4B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activator of S phase kinase like protein 1 ASK like protein 1 ASKL1 chifb Chiffon homolog B DBF 4B DBF4 homolog B (S. cerevisiae) DBF4 homolog B Dbf4 related factor 1 DRF1 Protein DBF4 homolog B ZDBF1B Zinc finger DBF type containing 1B DBF4B_HUMAN. |
| Product description | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. |
| Immunogen | KLH conjugated synthetic peptide derived from human DBF4B/ASKL1 41-140/615 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Activator of S phase kinase like protein 1 ASK like protein 1 ASKL1 chifb Chiffon homolog B DBF 4B DBF4 homolog B (S. cerevisiae) DBF4 homolog B Dbf4 related factor 1 DRF1 Protein DBF4 homolog B ZDBF1B Zinc finger DBF type containing 1B DBF4B_HUMAN. |
|---|---|
| Molecular Weight(MW) | 18 KDa |
| Cellular Localization | Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction. |
| SwissProt ID | Q8NFT6 |
|---|
Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti-DBF4B (ER1907-21) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18kD Observed band size: 18kD
Application
Fig2: Sample: Hela Cell (Human) Lysate at 30 ug Primary: Anti-DBF4B (ER1907-21) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18kD Observed band size: 18kD
Application
Fig3: Sample: U2os Cell (Human) Lysate at 30 ug Primary: Anti-DBF4B (ER1907-21) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18kD Observed band size: 18kD| Positive Control | Widely expressed. Highly expressed in testis. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
